Table 1. Antibodies for western blot analysis

Target	Host species / type	Dilution rate	Brand	Cat. no.
p-Akt	Rabbit IgG / primary	1:1,000	CST	4060
Akt	Rabbit IgG / primary	1:1,000	CST	4685
p-ERK	Rabbit IgG / primary	1:1,000	CST	4370
ERK	Rabbit IgG / primary	1:1,000	CST	4695
SHH	Rabbit IgG / primary	1:1,000	CST	2207
SUFU	Rabbit IgG / primary	1:1,000	CST	2520
Cyclin D3	Rabbit IgG / primary	1:1,000	CST	2936
GAPDH	Rabbit IgG / primary	1:10,000	CST	2118
Rabbit IgG	Mouse IgG-HRP /secondary	1:10,000	SCBT	SC-2357

p-Akt, phosphorylated protein kinase B; p-ERK, phosphorylated extracellular signal-regulated kinase; SHH, sonic hedgehog; SUFU, suppressor of fused homolog; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IgG, immunoglobulin G; HRP, horseradish peroxidase. CST, Cell Signaling Technology (Danvers, MA, USA); SCBT, Santa Cruz Biotechnology (Dallas, TX, USA).