Table 1. Primer sequences employed to investigate the impact on gene expression related to anti-inflammatory effects

Gene	Primer sequence (5’to 3’)	Accession no.
β-Actin	Forward: CCACAGCTGAGAGGGAAATC	NM_007393.5
β-Actin	Reverse: AAGGAAGGCTGGAAAAGAGC	NM_007393.5
IL-6	Forward: AGTTGCCTTCTTGGGACTGA	NM_031168.2
IL-6	Reverse: CAGAATTGCCATTGCACAAC	NM_031168.2
IL-1β	Forward: GGGCCTCAAAGGAAAGAATC	NM_008361.4
IL-1β	Reverse: TACCAGTTGGGGAACTCTGC	NM_008361.4
TNF-α	Forward: ATGAGCACAGAAAGCATGATC	NM_013693.3
TNF-α	Reverse: TACAGGCTTGTCACTCGAATT	NM_013693.3
COX-2	Forward: AGAAGGAAATGGCTGCATAA	NM_011198.5
COX-2	Reverse: GCTCGGCTTCCAGTATTGAG	NM_011198.5
iNOS	Forward: TTCCAGAATCCCTGGACAAG	NM_010927.4
iNOS	Reverse: TGGTCAAACTCTTGGGGTTC	NM_010927.4