Table 1 Optimum condition and characteristics of different commercial proteolytic enzymes

Commercial name	Origin	Optimum pH	Optimum temperature	Unit activity*	Reaction mechanism
Alcalase® 2.4 L	B. licheniformis	6.5-8.5	55-70	2.4 AU/g	endo
FlavourzymeTM 500 MG	A. oryzae	5.0-7.0	50	500 LAPU/g	endo, exo
Neutrase®	Bacillus amyloliiensquefaciens	5.5-7.5	45-55	0.8 AU/g	endo
ProtamexTM	Bacillus	5.5-7.5	35-60	1.5 AU/g	endo

^*AU: Anson Unit, LAPU: leucine aminopeptidase unit.