Table 2.
Operation conditions of HPLC instrument used for the vitamin B1 measurements.

Classification	Condition
Column	Luna IOu C18 (4.6×250 mm)
Mobile phase	MeOH : Ammonium acetate (3:7, V/V)
Flow rate	Main column: 0.7 mL/min, Reagent pump: 0.7 mL/min
Oven temperature	30°C
Injection volume	20 μL
Detector	474 Fluorescence Detector (Ex : 375 nm, Em : 450 nm)