Table 1.
Analytical conditions for the analysis of free sugars with HPLC.

Parameter	Analytical Conditions
HPLC	Waters 2690
Column	Shodex Asahipak NH2P-50 4E (300 mm×3.9 nm, 10 µm)
Flow rate	1.0 mL/min
Injection volume	20 µL
Detector	Waters 410 (RI Detector )
Column temperature	30°C
Mobile phase	Acetonitrile : H2O (75:25, v/v)